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Mark Failla
AP110
(614) 247-2412
mfailla@ehe.osu.edu
Professor, Department of Human Nutrition
Associate Dean for Research, College of Education and Human Ecology
View Mark Failla's curriculum vitae.
B.S. Chemistry, St. Francis College, Brooklyn, NY, 1970
M.S. Microbiology, Indiana University, Bloomington, IN, 1975
Ph.D. Microbiology, Indiana University,1976
It has been estimated that the human diet contains approximately 20,000 unique compounds, although far fewer than 100 of these are classified as essential for life. Epidemiological, laboratory and clinical studies consistently have revealed that many non-essential components of the diet are capable of modulating health. Moreover, there is a rapidly increasing literature demonstrating that many tissues, as well as the resident bacteria colonizing the large intestine, convert numerous ingested compounds to metabolites that affect the replication, differentiation and functions of various cell types. The identification of the bioactive compounds present in foods and the elucidation of their molecular mechanisms of action continues to attract much interest in the biomedical sciences. Insights are facilitating interactions among nutrition and food scientists, geneticists, plant and animal scientists, and physician/veterinary scientists, aimed at the development of new foods that promote health and can be used to treat diseases. This truly is an exciting time to be a nutrition scientist!
Our research team is interested in problems related to the digestive stability, intestinal cell uptake, absorption and functions of health promoting compounds in foods. Our current efforts primarily focus on two families of compounds in plant foods, viz., the carotenoids and soy isoflavonoids. We are particularly interested in screening provitamin A bioavailability in cultivars of staple foods (e.g., maize, cassava, rice and sweet potato) that represent the primary source of energy and nutrients for populations in poor rural areas of developing countries. We also are systematically addressing the influence of amount and type of dietary fat on the uptake of carotenoids and other fat soluble compounds in foods and meals. Finally, we are investigating the potential anti-inflammatory activities of various dietary compounds in the gut and the influence of such compounds on interactions between gut bacteria and intestinal epithelial cells.
We use in vitro approaches to simulate digestion and study the absorption, metabolism, and transepithelial transport of bioactive compounds by human intestinal cells, and employ cell and animal models, as well as human and animal intervention trials. We also collaborate with outstanding colleagues at OSU and other institutions to provide graduate students with opportunities to broaden their conceptual and technical skills.
Recent Publications
Chitchumroonchokchai, J. & Failla, M.L. (2006) Hydrolysis of zeaxanthin esters by carboxyl ester lipase during digestion facilitates micellarization and uptake of the xanthophyll by Caco-2 human intestinal cells. J. Nutrition 136: 588-594.
Ferruzzi, M.G., Lumpkin, J.L., Schwartz, S.J. & Failla, M.L. (2006) Digestive stability, micellarization and uptake of b-carotene isomers by Caco-2 human intestinal cells. J. Ag. Food Chem. 54: 2780-2785.
Terjesen B.F., Lee, K-J., Zhang, Y., Failla, M. & Dabrowski, K. (2006) Optimization of dipeptide-protein mixtures in experimental diet formulations for rainbow trout (Onchrhynchus mykiss) alevins. Aquaculture 254: 517-525.
Tian, Q., Failla, M., Bohn, T. and Schwartz, S. (2006) High-performance liquid chromatography atmospheric pressure chemical ionization tandem mass spectrometry determination of cholesterol uptake by Caco-2 cells. Rapid Comm. Mass Spectrometry. 20: 3056-3060.
Bhagavan, H.N., Chopra, R.J., Craft, N.E., Chitchumroonchokchai, C. & Failla, M.L. (2007) Assessment of Coenzyme Q absorption using an in vitro digestive/Caco-2 cell model. International J. Pharmaceutics, 333: 112-117.
Walsh, K.R., Haak, S.J., Bohn, T., Tian, Q., Schwartz, S.J. & Failla, M.L. Isoflavonoid glucosides are deconjugated and absorbed in the small intestine of human subjects with ileostomies. (2007) Am. J. Clin. Nutr. 85: 1050-1056.
Pullakhandam, R. & Failla, M.L. (2007) Micellarization and intestinal cell uptake of b-carotene and lutein from drumstick (Moringa oleifera) leaves. J. Medicinal Foods 10: 252-257.
Bohn, T., Tian, Q.C., Chitchumroonchokchai, C., Failla, M.L., Schwartz, S.J., Cotter, R., Waksman, J.A. (2007) Supplementation of test meals with fat-free phytosterol products can reduce cholesterol micellarization during simulated digestion and cholesterol accumulation by Caco-2 cells. J. Ag. Food Chem. 55: 267-272.
Thakkar, S., Failla, M.L. (2007) beta-carotene micellarization during in vitro digestion and uptake by Caco-2 cells is directly proportional to beta-carotene content in different genotypes of cassava. J. Nutr. 137: 2229-2233.
Huo, T., Ferruzzi, M.G., Schwartz, S.J., Failla, M.L (2007) Impact of fatty acyl composition and quantity of triglycerides on bioaccessibility of dietary carotenoids. J. Agr. Food Chem. 55: 8950-8957.
Winnick, J.J., W. Michael Sherman, W.M., Diane L. Habash, D.L.,Stout, M.B., Failla, M.L., Belury, M.A., Schuster, D.P. (2008) Short-term aerobic exercise training in obese humans with type 2 diabetes mellitus improves whole body insulin sensitivity through gains in peripheral, not hepatic insulin sensitivity. J Clin Endocrinol Metab 93: 771-778.
Failla, M.L., Chitchumroonchokchai, C., Ishida, B. (2008) In vitro micellarization and intestinal cell uptake of cis isomers of lycopene exceed those of all-trans lycopene. J. Nutrition 138: 482-486.
Long, A., Agler, A., Colitz, C., Failla, M., Bomser, J. (2008) Isolation and characterization of primary canine lens epithelial cells. Veterinary Ophthalmology 11: 38-42.
Ornelas-Paz, J., Failla, M.L. Yahia, E.L., Gardea-Bejar, A. (2008) The impact of stage of ripening and dietary fat on in vitro bioaccessibility of b-carotene in ‘Ataulfo’ mango. J. Agr. Food Chem. 56: 1511-1516.
Failla, M.L., Huo, T., Thakkar, SK. (2008) In vitro screening of relative bioaccessibility of carotenoids from foods. Asian Pacific J. Clin. Nutr. 17 (S1): 200-203.
Thakkar, S., Failla, M.L. (2008) Bioaccessibility of pro-vitamin A carotenoids is minimally affected by non-pro-vitamin A xanthophylls in maize (Zea mays sp.). J. Agr. Food Chem., 56: 11441-46.
Mills, J.P., Tumuhimbise, G.A., Jamil, K.M., Thakkar, S.K., Failla, M.L., Tanumihardjo S.A. (2008) Sweet potato beta-carotene bioefficacy is enhanced by dietary fat and not reduced by soluble fiber intake in Mongolian gerbils. J. Nutr. 139: 44-51.
Thakkar, S., Huo, T., Maziya-Dixon, B., Failla, M. (2009) Impact of style of processing on retention and bioaccessibility of ?-carotene in cassava (Manihot esculanta, Crantz). J. Agr. Food Chem. 57: 1344-1348.
Walsh, K.R., Haak, S.J., Fastinger, N.D., Bohn, T., Tian, Q., Mahan, D.C., Schwartz, S.J., Failla, M.L. (2009) Gastrointestinal metabolism and absorption of soy isoflavonoids in ileal canulated swine. Molecular Nutr. Food Res. 53: 277-286.
Bumrungpert, A., Kalpravidh, R.W., Suksamrarn, S., Chaivisuthangkura, A., Chitchumroonchokchai, C., Failla, M.L. (2009) Bioaccessibility, biotransformation and transport of ?-mangostin from Garcinia mangostana (Mangosteen) using simulated digestion and Caco-2 human intestinal cells. Molecular Nutr. Food Res. 53: in press.
He, J., Wallace, T., Keatley, K., Failla, M., Giusti, M.M. (2009) Stability of black raspberry anthocyanins in the digestive tract lumen and transport efficiency into gastric and small intestinal tissues in the rat. J. Agr. Food Chem. 57: in press.
Failla, M.L., Thakkar, S.K., Kim, J.Y. In vitro bioaccessibility of beta-carotene in orange fleshed sweet potato (Ipomoea batatas, Lam.). J. Agr. Food Chem. 57: in press.